Journal: International Journal of Molecular Sciences
Article Title: Augmentation of Bone Regeneration by Depletion of Stress-Induced Senescent Cells Using Catechin and Senolytics
doi: 10.3390/ijms21124213
Figure Lengend Snippet: Characteristics of sponges. Macroscopic ( A ) and field-emission scanning electron microscopic ( B ) images of sponges. LS-G, LPS sustained-release gelatin sponges; LS-G+EGCG, LS-G chemically modified with EGCG. ( C – E ) Evaluation of cytotoxicity of LS-G and LS-G+EGCG in vitro. The osteoblastic cell line UMR106 was treated with or without the sponges for three days. Control, without the sponges. ( C ) Live and dead cell staining. Green, live cells; red, dead cells. ( D ) Apoptosis analysis. Green, apoptotic cells; blue, DAPI (4′,6-diamidino-2-phenylindole) staining representing nuclei. ( E ) Cell counting kit (CCK-8) colorimetric assay. Data are expressed as mean ± standard deviation (SD), n = 4. N.S., not significant.
Article Snippet: Rat osteoblastic cell line UMR106 (CRL-1661, American Type Culture Collection, Manassas, VA, USA) was seeded in 48-well plates at a density of 1 × 10 4 /well and cultured in Dulbecco’s modified Eagle’s medium with 10% fetal bovine serum and 1% antibiotics.
Techniques: Modification, In Vitro, Control, Staining, Cell Counting, CCK-8 Assay, Colorimetric Assay, Standard Deviation